Bromobimane TCI B4220

Chemical Identity

CAS No.: 71418-44-5
PubChem: CID 114810·SID 253660146
Formula: C10H11BrN2O2
Molecular weight: 271.11 g/mol
Purity: >98.0%(HPLC)
Reference databases: ChEBI·ChemSpider·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 7-(bromomethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
SMILES: CC1=C(N2C(=C(C(=O)N2C1=O)C)CBr)C
InChIKey: AHEWZZJEDQVLOP-UHFFFAOYSA-N

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Bromobimane is a chemical compound widely used in laboratory settings, particularly in biochemical and molecular biology research. It functions as a highly effective fluorescent label, enabling the identification and visualization of specific molecules within biological samples. This compound is essential for researchers aiming to track molecular interactions or monitor cellular processes with high precision. Its utility spans across various experimental techniques, making it a versatile tool in modern laboratory workflows.

The compound is prized for its strong fluorescence properties and chemical stability, which allow it to maintain its integrity under diverse experimental conditions. Bromobimane's ability to bind to proteins and other biomolecules further enhances its applicability in structural and functional studies. This makes it an ideal choice for applications requiring high sensitivity and specificity. Its reliability and consistent performance contribute to its popularity among scientists working in life sciences.

In Indonesian laboratories, Bromobimane is commonly used in molecular biology, organic chemistry, and biomedical research. It plays a crucial role in protein labeling, cell analysis, and fluorescence-based assays. Its availability supports a wide range of research activities, ensuring that scientists can conduct experiments with confidence and accuracy. The compound's relevance in these fields underscores its importance in the scientific community.

Advantages (TCI brochure)

Blue fluorescence only when bonded to a thiol group
Specifically labeled to thiol groups

TCI brochures (PDF)

Fluorescent Labeling Dyes 2025-11-18 · p. 3

Related TCI products

Molecular Biology Research: Bromobimane is used for protein labeling and structural analysis due to its strong fluorescence and binding properties.
Fluorescence Microscopy: The compound enables visualization of cellular components and molecular interactions under a microscope.
Biochemical Assays: It serves as a fluorescent probe in assays that require detection of specific biomolecules or reactions.
Cell Imaging Studies: Bromobimane aids in tracking cellular processes and identifying specific molecules within living cells.
Biomedical Research: It is utilized in studies involving biological systems and molecular interactions relevant to disease mechanisms.

From the TCI brochure

Specific labeling of thiol groups

Brand	TCI
CAS number	71418-44-5
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	Available in various quantities as per standard laboratory requirements
Physical form	Solid or liquid, depending on the specific formulation
Storage	Store in a cool, dry place away from light and moisture

Data from the TCI brochure

λex,max	390 nm (saat terikat pada tiol)
λem,max	480 nm (saat terikat pada tiol)

Source: TCI brochure LL039 (2025-11-18)

Bromobimane should be stored in a cool, dry environment, away from direct light and moisture to maintain its stability and effectiveness. It is recommended to use airtight containers to prevent exposure to air and humidity, which could degrade the compound. Laboratory personnel should handle the material with care, using appropriate personal protective equipment such as gloves and safety goggles. Proper ventilation should be maintained in the workspace to minimize inhalation risks. The compound should be kept in a secure location, away from incompatible substances to ensure safety. Regular inspection of storage conditions is advised to ensure the integrity of the material remains uncompromised.

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