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TCI

CAS 298-93-1

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide TCI D0801

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide TCI D0801
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Description

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide, commonly known as MTT, is a vital chemical compound widely used in life sciences research. It serves as a key reagent in cell viability assays, enabling scientists to assess the metabolic activity of living cells. MTT is particularly valuable in biomedical and biotechnological applications where the health and functionality of cells are critical. Its ability to be reduced by mitochondrial enzymes makes it an essential tool for evaluating cellular responses in various experimental conditions.

MTT is favored for its chemical stability and solubility in organic solvents such as dimethyl sulfoxide (DMSO) and acetone. These properties make it easy to handle and incorporate into a wide range of biochemical protocols. Additionally, its non-toxic nature and high safety profile ensure that it can be safely used in diverse laboratory settings without posing significant risks to researchers or the environment. The compound’s consistent performance across different experimental conditions further enhances its reliability as a detection reagent.

In Indonesian laboratories, MTT is a staple in biomedical, pharmacological, and biotechnological research. It is commonly used to study the effects of drugs on human cells, monitor cellular responses to stimuli, and assess the overall health of cell cultures. Its versatility and effectiveness have made it an essential component in both academic and industrial research environments, supporting advancements in life sciences and related fields.

Laboratory Applications

  • Cell viability testing for evaluating the impact of drugs on human cell cultures.
  • Detection of mitochondrial activity to assess cellular metabolic health and function.
  • Monitoring cell responses to various stimuli in biotechnological and pharmacological studies.
  • Supporting research in immunology by measuring immune cell activation and proliferation.
  • Assisting in the development of new therapeutic agents through in vitro cell-based assays.

Specifications

  • Brand: TCI
  • CAS number: 298-93-1
  • Category: Life Science > Biochemicals and Reagents > Luminescent Compounds/Detection
  • Pack sizes: Available in standard laboratory quantities as per supplier specifications
  • Physical form: Powder
  • Storage note: Store in a cool, dry place away from light

Handling and Storage

MTT should be stored in a cool, dry environment, away from direct sunlight and sources of heat. It is recommended to keep the compound in a tightly sealed container to prevent moisture absorption and contamination. Due to its chemical stability, MTT does not require refrigeration but should be protected from prolonged exposure to air. When handling MTT, it is advisable to use appropriate personal protective equipment, such as gloves and safety goggles, to ensure laboratory safety. The compound is non-toxic but should be handled with care to avoid inhalation or contact with skin. Proper labeling and storage practices help maintain the integrity and effectiveness of the reagent in laboratory settings.

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