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Description
TCI D5799 N-Succinimidyl 7-(Diethylamino)coumarin-3-carboxylate is a fluorescent labelling reagent that combines two functional parts within a single molecule: a fluorescent 7-diethylaminocoumarin chromophore and a reactive N-hydroxysuccinimide (NHS) ester group. In life science laboratories, reagents of this kind act as "tags" that are covalently attached to biomolecules so that their presence and behaviour can be observed with fluorescence-based instruments. In this way, proteins, peptides, antibodies, or small amines that were previously invisible become detectable, quantifiable, and traceable within an experimental system.
The main property that makes this reagent a preferred choice is the reaction selectivity of the NHS ester toward primary amines. Under mildly basic aqueous conditions, this activated ester reacts to form a stable amide bond with the N-terminal amino group of a protein or with lysine side chains, without requiring any additional activating reagent. The coumarin core carrying a diethylamino substituent is a strong electron-donating group that produces the characteristic coumarin emission in the blue range, offering a detection channel distinct from green and red dyes and making it useful for labelling work where channel separation matters.
In Indonesian laboratories, this reagent is typically used in university research groups, biochemistry and molecular biology facilities, and analytical laboratories that carry out fluorescence-based detection. It is commonly applied where a research team needs to derivatise amine-containing compounds prior to instrumental analysis, or to prepare fluorescently labelled protein and peptide conjugates for downstream study. Because it is supplied as a research chemical, it is generally handled by trained personnel within controlled laboratory workflows rather than in routine production settings.
Laboratory Applications
- Protein and antibody labelling: the NHS ester reacts with lysine side chains and N-terminal amines to form stable amide bonds, producing fluorescently tagged conjugates for detection.
- Peptide derivatisation: small synthetic peptides carrying free amine groups can be tagged directly, giving them a fluorescent handle for tracking through purification and analysis steps.
- Fluorescent derivatisation for chromatographic analysis: amine-containing analytes that lack their own fluorescence are converted into detectable derivatives suitable for fluorescence detector systems.
- Multichannel fluorescence experiments: the blue emission of the diethylaminocoumarin core provides a detection channel separate from green and red dyes used in the same experiment.
- Amine quantification studies: because the reagent reacts selectively with primary amines, it supports work in which the amount of accessible amine groups must be measured or compared.
Specifications
- Brand: TCI
- CAS number: 139346-57-9
- Category: Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
- Product code: D5799
- Pack sizes: available in the pack sizes offered by the manufacturer for this catalogue item; please confirm the required size when ordering.
- Storage note: keep in a cool, dry, tightly closed container, protected from light and moisture, in accordance with the manufacturer's documentation.
Handling and Storage
Store the reagent in its original tightly closed container in a cool, dry place, protected from light and from moisture, since NHS esters are susceptible to hydrolysis when exposed to humidity. Amber glass vials or the supplied container are suitable for storage, and the container should be allowed to reach room temperature before opening to reduce condensation on the contents. Handle the material in a well-ventilated area or fume hood, wearing gloves, safety glasses, and a laboratory coat, and avoid contact with skin, eyes, and clothing. Prepare working solutions in anhydrous solvent shortly before use, keep the material away from incompatible substances, and follow the manufacturer's safety data sheet and institutional waste disposal procedures.
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