Brucine Sulfate Heptahydrate [for Nitrate Analysis] TCI B0672

Chemical Identity

CAS No.: 60583-39-3
PubChem: CID 24721400·SID 87563678
Formula: C46H54N4O12S
Molecular weight: 887.0 g/mol
Purity: >98.0%(HPLC)(T)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: bis((4aR,5aS,8aR,13aS,15aS,15bR)-10,11-dimethoxy-4a,5,5a,7,8,13a,15,15a,15b,16-decahydro-2H-4,6-methanoindolo[3,2,1-ij]oxepino[2,3,4-de]pyrrolo[2,3-h]quinolin-14-one);sulfuric acid
SMILES: COC1=C(C=C2C(=C1)[C@]34CCN5[C@H]3C[C@@H]6[C@@H]7[C@@H]4N2C(=O)C[C@@H]7OCC=C6C5)OC.COC1=C(C=C2C(=C1)[C@]34CCN5[C@H]3C[C@@H]6[C@@H]7[C@@H]4N2C(=O)C[C@@H]7OCC=C6C5)OC.OS(=O)(=O)O
InChIKey: HCMSIGALSOEZRW-WIMNQIPBSA-N

Rp 1.801.800,00

~~Rp 1.275.750,00~~ Rp 1.801.800,00

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TCI Brucine Sulfate Heptahydrate (product code B0672) is the sulfate salt of brucine, a naturally occurring alkaloid structurally related to strychnine. This grade is labelled "for Nitrate Analysis," so it is prepared specifically for determining nitrate content. In analytical laboratories, brucine sulfate is known as the reagent in the brucine method, a classical colorimetric procedure for measuring nitrate in water. In strongly sulfuric acid conditions, brucine reacts with nitrate to produce a yellow colour whose intensity is proportional to the nitrate concentration.

Analysts choose this reagent because its reaction is specific to nitrate and the colour it forms can be measured with a visible-light spectrophotometer, so no expensive instrumentation is needed. The sulfate heptahydrate salt form is easier to handle and dissolve than the free base, which makes reagent preparation more convenient. The dedicated nitrate-analysis grade matters because impurities can interfere with colour development and shift measured results, so a reagent prepared for this purpose supports more reliable readings. Beyond nitrate analysis, brucine is also known as a chiral resolving agent that separates enantiomers of organic acids by forming diastereomeric salts.

In Indonesia, nitrate monitoring is important for assessing the quality of drinking water, groundwater, and other water sources. Environmental testing laboratories, water utility quality-control units, university chemistry departments, and research institutions can use brucine sulfate for routine nitrate determination with the brucine colorimetric method. Its compatibility with standard visible spectrophotometers makes it practical for laboratories with modest equipment budgets. Teaching laboratories can also use it to demonstrate classical colorimetric analysis and the principles of chiral resolution in organic chemistry courses.

TCI brochures (PDF)

Alkaloids 2025-10-10 · p. 6

Related TCI products

Nitrate determination in water: the brucine method uses this reagent in concentrated sulfuric acid to form a yellow colour whose intensity is proportional to nitrate concentration.
Drinking water and groundwater quality monitoring: the nitrate-analysis grade reduces interference from impurities, which helps environmental and utility laboratories obtain consistent and dependable nitrate readings.
Spectrophotometric analysis with basic instrumentation: the coloured product is measured in the visible range, so laboratories can run nitrate tests on a standard visible spectrophotometer instead of costly instruments.
Chiral resolution of organic acids: brucine forms diastereomeric salts with racemic organic acids, which allows chemists to separate the enantiomers through differences in salt properties.
Analytical chemistry education: the classical brucine colorimetric method gives students a clear, hands-on example of reagent-based colour formation and quantitative measurement using absorbance.

Brand	TCI (product code B0672)
CAS number	60583-39-3
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Alkaloids
Pack sizes	please check the product page or contact AMI Scientific for available pack sizes, and follow the storage conditions on the TCI label and SDS
Physical form	sulfate heptahydrate salt of brucine; please confirm appearance on the TCI certificate of analysis
Storage	—

Grade: for Nitrate Analysis

Store brucine sulfate heptahydrate in its original tightly closed container in a cool, dry, well-ventilated area, away from direct sunlight, moisture, and incompatible substances such as strong oxidising agents. Because brucine is a toxic alkaloid related to strychnine, keep it in a secured, clearly labelled location where only trained personnel can access it. Wear gloves, safety glasses, and a lab coat, and weigh it in a fume hood or ventilated enclosure so you do not inhale dust or swallow it. The brucine method uses concentrated sulfuric acid, so take the usual precautions for corrosive acids. Wash your hands after handling, never eat or drink in the work area, and dispose of residues and waste solutions according to the SDS and local hazardous waste regulations.

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