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CAS 57282-61-8

Hyaluronate Tetrasaccharide TCI H1284

Hyaluronate Tetrasaccharide TCI H1284

Chemical Identity

CAS No.
57282-61-8
PubChem
CID 44630032·SID 87561693
Formula
C28H44N2O23
Molecular weight
776.6 g/mol
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
(2R,3S,4S,5S,6R)-6-[(2S,3S,4R,5S,6S)-3-acetamido-2-[(2R,3S,4R,5S,6R)-6-[(3S,4R,5S,6S)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic
SMILES
CC(=O)N[C@H]1[C@H]([C@@H]([C@@H](O[C@H]1O[C@H]2[C@@H]([C@@H]([C@@H](O[C@H]2C(=O)O)O[C@H]3[C@@H]([C@@H](OC([C@H]3NC(=O)C)O)CO)O)O)O)CO)O)O[C@H]4[C@H]([C@H]([C@@H]([C@@H](O4)C(=O)O)O)O)O
InChIKey
KIUKXJAPPMFGSW-NSFQZMNSSA-N
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Hyaluronate Tetrasaccharide is an oligosaccharide derived from the breakdown of hyaluronic acid, composed of four sugar units arranged as two alternating pairs of glucuronic acid and N-acetylglucosamine. As a defined fragment of a naturally occurring glycosaminoglycan, it occupies an important position in glycoscience because it provides a structure of exact, known length, unlike high molecular weight hyaluronic acid, which is always a polydisperse mixture. In the laboratory, fragments of this kind are used as analytical standards, as model ligands, and as test materials in studies of carbohydrate–protein interactions.

The principal advantage of this material is structural certainty. Because the number of sugar units and their sequence are precisely known, researchers can correlate a biological response or an analytical signal directly with a specific chain length — something that is impossible to achieve with heterogeneous polymers. Hyaluronan oligosaccharides are also strongly hydrophilic and negatively charged owing to the carboxylate groups on the glucuronic acid residues, so they dissolve readily in water and in buffer solutions and are well suited to both chromatographic analysis and mass spectrometry. Short fragments such as the tetrasaccharide are known to behave differently from their long-chain counterparts, which is precisely why they are studied separately.

In Indonesian laboratories, this material is typically used in academic and institutional research settings working on glycoscience, biochemistry, and biomaterials. It serves research groups that require a reference oligosaccharide of defined length for method development, for calibrating analytical procedures, or for comparative studies against polydisperse hyaluronic acid preparations. Because only small quantities are normally required for such work, it is generally ordered as a research-scale reagent rather than in bulk.

  • Analytical standard preparation — the defined four-unit chain length provides a reliable reference point for calibrating chromatographic and mass spectrometric methods used in hyaluronan analysis.
  • Carbohydrate–protein interaction studies — as a model ligand of exactly known structure, it allows binding behaviour to be attributed to a specific oligosaccharide length rather than to an undefined polymer population.
  • Glycosaminoglycan fragment research — it supports investigations into how short hyaluronan fragments differ functionally from high molecular weight hyaluronic acid in biological systems.
  • Chromatographic method development — its high water solubility and negative charge make it well suited to developing and validating separation methods for charged oligosaccharides.
  • Mass spectrometry reference work — the precisely defined composition and sequence allow fragmentation patterns to be interpreted with confidence during structural characterisation studies.

Brand TCI
CAS number 57282-61-8
Molecular formula —
Purity —
Category Chemistry > Chemical Biology > Glycoscience
Pack sizes available in research-scale packaging; please refer to the current catalogue listing for available options
Physical form —
Storage refer to the manufacturer's stated storage conditions on the product label and certificate of analysis
  • Catalogue Number: H1284

Data from the TCI brochure

Rumus dan berat molekulC28H44N2O23 = 776.65
Nomor CAS[57282-61-8]
PelarutD2O
Standar internalt-BuOH (δ 1.23)
Suhu pengukuran24.1 ℃ (NMR dalam D2O)

Source: TCI brochure reagent_for_glyco_chemistry_&_biology_5th_ed ()

Store the material according to the storage conditions specified by the manufacturer on the product label. As with other oligosaccharide preparations, the container should be kept tightly closed and protected from moisture, since the strongly hydrophilic nature of hyaluronan fragments makes them prone to absorbing atmospheric water. Use clean, dry spatulas and glassware when handling, and allow the container to reach room temperature before opening in order to avoid condensation on the contents. Prepare solutions in purified water or an appropriate buffer, and observe standard laboratory practice: wear gloves, safety glasses, and a laboratory coat, and consult the safety data sheet before first use.

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