Collection: Copper-free Click Reaction Reagents

Copper-free Click Reaction Reagents — contains reagents based on trans-cyclooctene (TCO) and their conjugated derivatives with PEG, biotin, maleimide, or DBCO that react rapidly without a metal catalyst. This group supports bioorthogonal reactions for labeling molecules in biological systems.

Copper-free Click Reaction Reagents are used by bioconjugation researchers to label proteins, antibodies, or cell surfaces through rapid reactions with tetrazine partners without requiring a copper catalyst that can damage biomolecules. The TCO-PEG3-DBCO derivative allows two types of click reactions to be combined at once, while the biotin and maleimide variants facilitate detection or linking in imaging and target capture experiments.

Example products in this collection: TCI T4455 TCO-NHS equatorial isomer, TCI B6215 1426827-79-3 (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate (2mg*5), TCI T4262 TCO*-PEG3-NHS equatorial isomer, TCI B6275 1426827-79-3 (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate, TCI T4261 TCO*-PEG3-Maleimide equatorial isomer, TCI B6476 1448421-61-1 Nepsilon-5-Norbornene-2-yloxycarbonyl-L-lysine Hydrochloride (endo- and exo- mixture), TCI T4260 TCO*-PEG3-DBCO equatorial isomer, TCI B7003 1-(tert-Butyl)-3-(methylthio)-5-phenyl-1,2,4-triazin-1-ium Trifluoromethanesulfonate.

Pay attention to the geometric isomers (equatorial/axial) of trans-cyclooctene, as they affect reaction speed, as well as the PEG3 linker length and the type of terminal reactive group, which must match the conjugation target. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under Bioconjugation, often used together in one laboratory workflow:

• PEGylation Reagents and PEG Spacers
• Biotins and Streptavidins
• Functionality Creating Reagents
• Zero-length Crosslinkers
• Functional Group Blocking Reagents
• N-Terminal Cysteine Residue-specific Conjugation Reagents

Back to Bioconjugation. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official TCI Japan distributor in Indonesia — for availability checks and lead time directly from Japan.