Collection: Phosphorylating Agents and Phosphorothioating Agents [Nucleic Acid Synthesis]

Phosphorylating Agents and Phosphorothioating Agents [Nucleic Acid Synthesis] — contains phosphoramidite, pyrophosphate, and aromatic disulfide compounds that function in forming phosphodiester and phosphorothioate bonds in the sugar-phosphate backbone of nucleic acids. These reagents support key steps in oligonucleotide synthesis through phosphoramidite chemistry.

These phosphorylating agents and phosphorothioating agents are used by molecular biology and nucleic acid chemistry researchers in solid-phase oligonucleotide synthesis, including the capping, oxidation, and sulfurization steps to produce phosphorothioate bonds that are more stable against enzymatic degradation. Stearyl phosphoramidite derivatives and tributylammonium pyrophosphate are commonly used as reagents for modifying the 5' end of synthetic oligonucleotides.

Example products in this collection: TCI T4256 207273-84-5 5'-Stearyl Phosphoramidite, TCI B6655 158041-82-8 6-[Bis(4-methoxyphenyl)phenylmethoxy]hexyl 2-Cyanoethyl N,N-Bis(1-methylethyl)phosphoramidite, TCI T4141 5975-18-8 Tributylammonium Dihydrogen Diphosphate, TCI B6656 158665-27-1 12-[Bis(4-methoxyphenyl)(phenyl)methoxy]dodecyl 2-Cyanoethyl N,N-Diisopropylphosphoramidite, TCI B5440 857447-79-1 Bis(tetrabutylammonium) Dihydrogen Pyrophosphate, TCI B6709 125607-09-2 1,1-Bis(4-methoxyphenyl)-1-phenyl-2,5,8,11,14,17-hexaoxanonadecan-19-yl 2-Cyanoethyl N,N-Diisopropylphosphoramidite, TCI B3623 15088-78-5 Bis(phenylacetyl) Disulfide, TCI C0976 15074-54-1 2-Chlorophenyl Phosphorodichloridate [Phosphorylating Agent].

Please note the compatibility of the reagents with the phosphoramidite synthesis cycle used, and maintain moisture-free storage conditions given the sensitivity of phosphorus groups to hydrolysis. All products are genuine products of Tokyo Chemical Industry (TCI) Japan, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under Nucleic Acid Chemistry, often used together in a single laboratory workflow:

• Coupling Activators and Condensing Agents [Nucleic Acid Synthesis]
• Protecting Agents for Hydroxy and Amino Groups [Nucleic Acid Synthesis]
• Nucleoside Phosphoramidites [Nucleic Acid Synthesis]
• Peptide Nucleic Acid (PNA) Monomers
• Sulfur Transfer Reagents [Nucleic Acid Synthesis]

Back to Nucleic Acid Chemistry. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — the official distributor of TCI Japan in Indonesia — to check availability and lead time directly from Japan.