Collection: Coupling Activators and Condensing Agents [Nucleic Acid Synthesis]

Coupling Activators and Condensing Agents [Nucleic Acid Synthesis] — includes coupling activator reagents and condensing agents such as sulfonyl imidazole derivatives, tetrazoles, and pyridinium salts used to form phosphodiester or phosphotriester bonds in oligonucleotide synthesis. These compounds activate phosphate groups so as to facilitate the formation of internucleotide bonds during the assembly of oligonucleotide chains.

These activator and condensing reagents are used by molecular biology and nucleic acid chemistry laboratories in the coupling step of oligonucleotide synthesis based on phosphotriester or phosphoramidite methods using solid-phase synthesis. For example, tetrazole and sulfonyl imidazole derivatives activate phosphite or phosphate groups so that they react rapidly with the hydroxyl group of the next nucleoside, while pyridinium trifluoroacetate salts are often used as a buffer or weak acid catalyst in the deprotection and neutralization steps.

Example products in this collection: TCI T1985 2232-08-8 1-(p-Toluenesulfonyl)imidazole, TCI B6329 50847-09-1 2-Bromo-1H-imidazole-4,5-dicarbonitrile, TCI T1410 50257-40-4 1-(2,4,6-Triisopropylbenzenesulfonyl)imidazole, TCI B6330 99257-95-1 1H-Benzimidazol-3-ium Trifluoromethanesulfonate, TCI B3020 21871-47-6 5-(Benzylthio)-1H-tetrazole, TCI C2325 41864-22-6 1,1'-Carbonyldi(1,2,4-triazole), TCI T0459 6553-96-4 2,4,6-Triisopropylbenzenesulfonyl Chloride, TCI C2421 434937-12-9 1-(Cyanomethyl)piperidinium Tetrafluoroborate.

Consider the compatibility of the activator with the synthesis method used (phosphotriester or phosphoramidite), the purity grade, and storage stability, since some of these reagents are sensitive to air humidity and require airtight containers. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other parallel categories under Nucleic Acid Chemistry, often used together in a single laboratory workflow:

• Phosphorylating Agents and Phosphorothioating Agents [Nucleic Acid Synthesis]
• Protecting Agents for Hydroxy and Amino Groups [Nucleic Acid Synthesis]
• Nucleoside Phosphoramidites [Nucleic Acid Synthesis]
• Peptide Nucleic Acid (PNA) Monomers
• Sulfur Transfer Reagents [Nucleic Acid Synthesis]

Back to Nucleic Acid Chemistry. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official distributor of TCI Japan in Indonesia — for availability checks and lead times directly from Japan.