Amino Acid Sequencing Reagents

Amino Acid Sequencing Reagents — includes solvents, acids, and derivatization reagents such as pyridine, acetic acid, as well as trifluoroacetic acid and heptafluorobutyric acid used in the Edman degradation steps for protein and peptide sequencing. This group supports repeated reaction cycles from coupling to cleavage of amino acid residues.

These reagents are used by protein biochemistry researchers in automated sequencers to determine the sequence of amino acid residues from the N-terminus of a protein or peptide through repeated reactions with phenyl isothiocyanate. Solvents such as ethyl acetate and pyridine play a role in the extraction and washing steps of the phenylthiohydantoin derivatives formed in each degradation cycle.

Example products in this collection: TCI Q0034 110-86-1 Pyridine [Sequencing Solvent], TCI C0142 109-69-3 1-Chlorobutane, TCI A5513 103-72-0 Phenyl Isothiocyanate [for HPLC Labeling], TCI P0364 5624-13-5 Phenylthiohydantoin-aspartic Acid, TCI A2035 64-19-7 Acetic Acid, TCI P0366 4370-90-5 Phenylthiohydantoin-methionine, TCI Q0054 375-22-4 Heptafluorobutyric Acid [Sequenation Reagent], TCI P0367 4332-97-2 Phenylthiohydantoin-phenylalanine.

Please pay attention to the analytical grade (sequencing grade) that is free of contaminants that interfere with detection, as well as the suitability of the solvent with the automated sequencer instrument being used. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other parallel categories under Application Specific Reagents, often used together in a single laboratory workflow:

Back to Application Specific Reagents. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official distributor of TCI Japan in Indonesia — for availability checks and lead times directly from Japan.

Related TCI catalogs (PDF)

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