Collection: Fluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]

Fluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents] — contains fluorescence labeling reagents such as NBD-F, NBD-Cl, and DBD-F that react with both primary and secondary amino groups to form fluorescent derivatives. This category is specifically designed for derivatization prior to HPLC analysis to improve the detection sensitivity of compounds that are difficult to detect directly.

Fluorescence Detection Labeling Reagents for Amino Groups are used by analytical researchers to tag amino acids, peptides, and other amines before HPLC separation, so they can be detected by fluorescence with high sensitivity. NBD-F and NBD-Cl are commonly used for derivatization of primary amino groups, 2,3-Naphthalenedialdehyde reacts specifically with primary amines to form fluorescent derivatives, while (S)-(+)-NBD-Py-NCS is used for enantiomeric purity (e.e.) determination through the formation of diastereomer pairs.

Example products in this collection: TCI A5595 98358-90-8 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling], TCI D0005 605-65-2 Dansyl Chloride (10% in Acetone), TCI A5594 7149-49-7 2,3-Naphthalenedialdehyde [Fluorimetric Reagent for Primary Amines], TCI D0656 605-65-2 Dansyl Chloride, TCI A5593 29270-56-2 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling], TCI D1669 29711-79-3 4-Dimethylamino-1-naphthyl Isothiocyanate [for HPLC Labeling], TCI A5592 10199-89-0 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling], TCI F0026 3326-32-7 Fluorescein 5-Isothiocyanate (isomer I).

Selection should consider derivatization reaction conditions such as pH and reaction time, the excitation-emission wavelengths of the fluorescent products, and the suitability of the reagent for the target amino group type. All products are genuine products of Tokyo Chemical Industry (TCI) Japan, with purity, packaging, and storage conditions listed on the page of each item.

Other parallel categories under HPLC Derivatization Reagents, often used together in one laboratory workflow:

• UV Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Carboxy Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]
• UV Detection Labeling Reagents (for Carboxyl Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]

Back to HPLC Derivatization Reagents. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — the official TCI Japan distributor in Indonesia — to check availability and lead time directly from Japan.